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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Transfusion. 2014 Jul 20;54(12):3186–3197. doi: 10.1111/trf.12755

Figure 4. Depletion of macrophages in vivo reduces circulating MCP-1 levels in response to refrigerator-stored RBC transfusions. Refrigerator-stored RBC transfusions induce the highest levels of MCP-1 mRNA synthesis in the spleen.

Figure 4

Transfusion recipients were male C57BL/6 mice. (A) Leukoreduced fresh and 14-day refrigerator-stored (“Old”) RBCs were separately transfused into recipients previously infused intraperitoneally with liposomes containing either PBS or clodronate. The recovery of the transfused RBCs was calculated by dual-label flow cytometry at 24 hours post-transfusion. Results are presented as mean ± s.d.

Mice were infused intraperitoneally with liposomes containing either PBS or clodronate and then infused with PBS or transfused with fresh or 14-day refrigerator-stored RBCs. Mice were sacrificed 2 hours after infusion/transfusion and circulating MCP-1 levels (B) and KC levels (C) were quantified.

Mice were transfused with fresh or 14-day refrigerator-stored RBCs. Two hours after transfusion, organs were harvested and MCP-1 expression (D) and KC expression (E) were measured by a real-time qPCR primer assay. The fold increases in cytokine expression after stored RBC transfusions were calculated for all organs by normalizing to the kidney results after fresh RBC transfusions. *P<0.05, ***P=0.001 compared with fresh RBCs. Shown are representative examples of at least 2 experiments.