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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Transfusion. 2014 Jul 20;54(12):3186–3197. doi: 10.1111/trf.12755

Figure 5. CD11b+ cells are responsible for increases in splenic MCP-1 mRNA expression following transfusions of refrigerator-stored RBCs.

Figure 5

Mice were infused with PBS, or transfused with fresh or 14-day refrigerator-stored (“Old”) RBCs. Two hours after transfusion, spleens were harvested and splenocytes separated into CD11b− and CD11b+ cell subpopulations by magnetic cell sorting. MCP-1 expression was measured as in Figure 3, Panel C. (A) In un-separated splenocytes, CD11b+ cells represent 6.2% of all cells (based on flow cytometry). In this population, MCP-1 mRNA levels increased 46-fold after refrigerator-stored RBC transfusions, as compared to the PBS control. (B & C) In CD11b− and CD11b+ subpopulations, respectively, MCP-1 mRNA levels increased 8- and 474-fold, respectively, after refrigerator-stored RBC transfusions, as compared to the PBS control. Shown are representative examples of at least 2 experiments.