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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Transfusion. 2014 Jul 20;54(12):3186–3197. doi: 10.1111/trf.12755

Figure 6. Refrigerator-stored RBC transfusions increase mRNA expression from the MCP-1 promoter in splenic CD11b+ cells of Ccl2-GFP reporter mice.

Figure 6

Ccl2-GFP reporter mice were transfused with fresh or 14-day refrigerator-stored (“Old”) RBCs. Splenocytes were analyzed by flow cytometry 2 hours after transfusion. (A) Transfusions of refrigerator-stored RBCs, as compared to fresh RBCs, increased the number of splenocytes with GFP expression driven by the MCP-1 promoter from 0.2 to 2.6%. (B) Splenocytes were stained for various cell surface markers, as indicated. Shown are histograms for the indicated markers gated on the splenocytes from the dashed rectangle in Panel A following transfusions with 14-day refrigerator-stored RBCs. Gray histograms: isotype-control antibodies; white histograms: test antibodies. Shown are representative examples of 2 experiments.