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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Neuroscience. 2014 Oct 24;0:555–565. doi: 10.1016/j.neuroscience.2014.10.033

Figure 4.

Figure 4

Calbindin immunohistochemistry demonstrates Purkinje cell death and loss at 24 hours. (A-C). Representative images showing labeling of Purkinje cells with anti-Calbindin D28K antibody. A continuous layer is observed in control (A.). Following cardiac arrest clear gaps, indicative of cell loss are observed at 24 hours (B.) and 7 days (C.) after CA/CPR. GCL: granule cell layer; PCL: Purkinje cell layer; ML: molecular layer. Scale bar = 50um. (D.) Quantification of Purkinje cell density analyzed at various times after CA/CPR. Loss of Purkinje cells is observed as early as 24 hours and is maximal at 7 days. Bars represent mean±SEM. N = 5-8. One-way ANOVA with Tukey’s multiple comparisons.