Extended Data Figure 8. Reduced proIL-1β expression and IL-1β maturation in neutrophils isolated from HFD-fed Pstpip2^cmo mice.

a, WT, Pstpip2^cmo and Pstpip2^cmoxIl1β^−/− bone marrow-derived macrophages were left untreated (UT) or were first primed with LPS for 3 hrs followed by additional stimulation with ATP (30 mins) or silica (12 hours) and IL-1β processing was evaluated by Western blot. Data are representative of three independent experiments. b, Western blotting for proIL-1β in untreated neutrophils that were purified from WT, LFD-fed Pstpip2^cmo and HFD-fed Pstpip2^cmo mice. Data are representative of two independent experiments. b-c, Neutrophils (b) or macrophages (c) from WT, LFD-fed Pstpip2^cmo and HFD-fed Pstpip2^cmo mice were left untreated (UT), or primed with LPS for 3 hrs and then stimulated with ATP (30 min) or silica (12 hours) and IL-1β processing was evaluated by Western blotting. Data are representative of two independent experiments.