Extended Data Figure 9. Depletion of neutrophils in anti-Ly6G treated Pstpip2^cmo mutant mice.

WT and Pstpip2^cmo mice received either PBS or 500 μg/mouse anti-Ly6G antibody (clone IA8) by intraperitoneal injection every 4-5 days starting at 6 weeks of age. a-c, Two weeks following the first anti-Ly6G treatment, FACS analysis was performed on peripheral blood leukocytes. a, Representative FACs plots of Gr-1 and CD11b expression on CD45.2⁺ gated cells. b, Enumeration of CD45.2⁺Gr-1^hiCD11b⁺ neutrophils in equal volumes of peripheral blood. c, Numbers of T cells (CD45.2⁺TCRβ⁺), CD45.2⁺Gr-1^-CD11b⁺ monocytes/macrophages and CD45.2⁺Gr-1^loCD11b⁺ cells in equal volumes of peripheral blood. Each point represents an individual mouse and the line represents the mean ± s.e.m. (***P < 0.001; Student’s t-test)