Extended Data Figure 1. Placing Pstpip2^cmo mice on a high fat and cholesterol diet limits the development of inflammatory bone disease.

a-e, Wild-type (WT) and Pstpip2^cmo mutant mice were fed a lean fat diet (LFD) or a high fat and cholesterol diet (HFD). Representative hind paw images (a) and representative pictures of popliteal lymph nodes (b) from WT, LFD Pstpip2^cmo and HFD Pstpip2^cmo mice at 12-14 weeks of age. c-d, Hematoxylin and eosin (H&E) staining (c) and pathology scores (d) of tail samples from 12-14 week old WT, LFD Pstpip2^cmo and HFD Pstpip2^cmo mice. Pathology scores were assigned in a blinded fashion by a veterinary pathologist based on the extent and severity of inflammation, osteolysis and osteogenesis. e, Representative immunostaining of neutrophils and macrophages in hind paw sections from 14-18 weeks old Pstpip2^cmo mice that were fed either a LFD or a HFD. (***P < 0.001; Student’s t-test)