Extended Data Figure 4. Dietary- and microbiota-associated factors influence the production of proIL-1β.

a, Footpad homogenates of 12-16 weeks old WT, LFD-fed Pstpip2^cmo and HFD-fed Pstpip2^cmo mice were immunoblotted for IL-1β. Data are representative of three independent experiments. b, Footpads samples were harvested from 10-14 week old specific pathogen free (SPF) WT, germ-free (GF) WT and Pstpip2^cmoxIl1β^−/− mice and proIL-1β protein levels were determined by Western blotting. c, CD45⁺ cells were isolated from the colons of GF WT mice and cells were left untreated (UT) or stimulated with LPS for 1 hr. Relative proIl1β mRNA expression levels were determined by qRT-PCR. Two biological replicates, with two technical replicates each.