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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: Neuroscience. 2014 Oct 13;0:165–170. doi: 10.1016/j.neuroscience.2014.10.002

Figure 1. Expression levels of FosB gene products.

Figure 1

A) Scheme showing that the FosB gene produces two mRNAs, FosB and ΔFosB. ΔFosB mRNA arises from alternative splicing within Exon 4 of the FosB primary transcript; this splicing generates a premature stop codon and the truncated ΔFosB protein. Δ2ΔFosB is generated from an alternative start codon corresponding to aa 79 in ΔFosB. The Δ1 and Δ3 ATGs, also present in the ΔFosB mRNA sequence, are not used as alteratnive start sites (Chen et al., 1997). The position of the BZIP domain present in all FosB gene products is shown. B) Western blotting of FosB gene products in NAc after repeated administration of cocaine (C, 15 mg/kg i.p., n=4–5) or saline (S) for 7 days, with mice analyzed 2 hr after the last injection. C) Western blotting of FosB gene products in NAc in bitransgenic mice that overexpress ΔFosB (Kelz et al., 1999), and to a much lesser extent Δ2ΔFosB, in the absence of doxycycline (DOX) (n=3–6). Histone H3 or GAPDH (glyceraldehyde 3-phosphate dehydrogenase), which are not regulated under the experimental conditions used, were utilized as a loading control. *p<0.05 by t-tests.