Table 1.

Composition of reagents for IP setup.^c

	Active Motif recommendations
Compontents	< 60 µl of chromatin	> 60 µl of chromatin	Actual
Protein G Magnetic Beads (µl)	25	25	25
ChIP Buffer 1 (µl)	10	20	20
Sheared Chromatin (µl)	20–60	61–150	130
20X PIC (µl)	1	2	2
dH2O (µl)	variable	variable	20
Antibody (µg)	1–3	1–3	3
Total Volume (µl)	100	200	200

^bActive Motif recommends these parameters for IP; we outlined the actual amounts that we used for our protocol.