Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Methods. 2014 Sep 8;70(0):89–96. doi: 10.1016/j.ymeth.2014.08.018

Table 2.

Comparison of DNA yield (ng/µl) for commercial and in-house buffersa.

Time
Min.		 Active Motif
Buffer (average)		 St. Dev. In-House Buffer
(average)		 St. Dev.
(+/−)
1 0.434 0.195 1.663 0.878
2.5 0.522 0.315 1.280 0.485
10 0.406 0.054 1.470 0.339
a

- Two buffers, one provided by Active Motif, Elution BufferAM2 and Reverse Cross-linking Buffer and one made in-house 1: 100 mM NaHCO3 and 2: 0.5% SDS, and in-house buffer 2: 0.3125 M NaCl were compared. Each set of time points is from the same donor, but three different donors were used to get multiple biological replicates for each buffer.