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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Invest Dermatol. 2014 Aug 9;135(1):84–93. doi: 10.1038/jid.2014.289

Figure 1. Deep tissue imaging by multiphoton microscopy demonstrates that spatial distribution of dermal leukocytes differ not only in depth but also between sites.

Figure 1

(a) Intravital imaging of ear skin of Cxcr6+/gfp and Csf1r-EGFP (MacGreen) mice at various depths showing GFP+ cells (green) in the deep dermis (arrowheads), extracellular matrix in the dermis (SHG signals, blue) and blood vessels (Evans blue, red). Cartilage is visualized through autofluorescence (asterisk) (representative images from n = 2 mice per strain). Scale bar 50 μm. (b) Calculated densities of dermal dendritic cells (DDC), mast cells (MC), T cells and macrophages (Mac) at different sites, stratified by distance from the dermo-epidermal junction (DEJ). n = 3 for all strains; 6–12 fields of view per site, with flow cytometric adjustment for fluorescence expression with at least 12 mice per strain.