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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Chem Phys Lipids. 2014 Sep 16;0:25–29. doi: 10.1016/j.chemphyslip.2014.09.001

Fig. 2. Effect of stereoisomers of cholesterol on dipole potential of membranes.

Fig. 2

Dipole potential in POPC membranes plotted with increasing concentrations of cholesterol (■), ent-cholesterol (▲), and epi-cholesterol (●). Data points shown are means ± S.E. of at least three independent measurements. The ratio of di-8-ANEPPS to total lipid was 1:100 (mol/mol) and total lipid concentration was 0.43 mM. Measurements were carried out at room temperature (~23°C). Lines joining the data points are provided merely as viewing guides. The structure of voltage-sensitive probe di-8-ANEPPS is shown in the upper left side. See section 2 for details.