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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Hypertension. 2014 Nov 3;65(1):153–160. doi: 10.1161/HYPERTENSIONAHA.114.04038

Figure 5.

Figure 5

Colocalization and co-immunoprecipitation of D4 and AT1 receptors in WKY and SHR RPT cells.

A. Colocalization of D4 and AT1 receptors in WKY and SHR RPT cells. The cells were washed, fixed, and immunostained for D4 receptor and AT1 receptor, as described in the Methods. Colocalization appears as yellow after merging the images of FITC-tagged D4 receptor (green) and rhodamine-tagged AT1 receptor (red).

B. Co-immunoprecipitation of D4 and AT1 receptors in WKY and SHR RPT cells. The cells were immunoprecipitated with D4 receptor antibodies and immunoblotted with AT1 receptor antibodies (*P<0.05 vs. WKY, n=3). One immunoblot (43 kDa) is depicted in the inset: (PC = positive control, NC = negative control). For the positive control, AT1 receptor antibody was used and for the negative control, IgG was used instead of D4 receptor antibody as the immunoprecipitant.