Figure 3.
Impact of RAGE ablation on tumor inflammatory response. A, Infiltration of CD11b+ cells (microglia and macrophages) into GL261 and K-Luc gliomas two weeks after intracranial implantation into WT or Ager−/− CX3CR1GFP mice. B, Representative flow cytometry contour plots (top) and quantification (bottom) of tumor-associated leukocytes in two-week old intracranial GL261 and K-Luc tumors in WT or Ager−/− mice. (Lymphocytes: CD45+, CD11b-, microglia: CD45intermediate, CD11bhigh, macrophages: CD45+, CD11bhigh. n=3 mice/group ± SD). C, qPCR of pro-inflammatory cytokines in normal brain (NB), and intracranial GL261 and K-Luc tumors two weeks after implantation. (n=4 mice/group ± SD), *: p<0.05. Experimental results are representative of three separate experiments.