Figure 7.

RAGE ablation in TAMs abrogates angiogenesis in GL261 gliomas. A, To demonstrate the feasibility of generating chimeric mice, bone marrow (BM) cross transplant experiments between wild-type (WT) and CX₃CR ^GFP₁ mice were performed. After recovery, mice were implanted with GL261 tumors and analyzed by histochemistry and flow cytometry. Glioma macrophages were identified as CD45^high CD11b^high cells (green cells and green events in dot plot) and appeared to infiltrate throughout the tumors (left panel). In the reverse transplantation experiments (right panel) where recipient mice were CX₃CR ^GFP₁, tumor microglia were identified as CD45^intermediate CD11b^high cells (green events and cells) that mostly remained within the margin of the tumor. B, qPCR demonstrating suppression of VEGFα expression in GL261 tumors that were implanted into WT mice transplanted with Ager^−/− BM. C, Tumor vessel characteristics in GL261 gliomas implanted into chimeric mice demonstrating normalization of dialated vessels in mice with either WT microglia or WT macrophages. Representative data from two separate experiments is shown. (n= 3 ± SD). *: p<0.05.