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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Cancer Res. 2014 Nov 6;74(24):7395–7405. doi: 10.1158/0008-5472.CAN-14-0993

Figure 1.

Figure 1

Mammary-specific transgene expression in MMTV-rtTA-TetO-PELP1 bitransgenic mice. A, Representative images of observed in vivo luminescence from bitransgenic mice fed with doxycycline-added drinking water or normal drinking water. Mice were injected ip of 150 mg/kg D-luciferin and after 10 min imaged on the IVIS imaging system. B, PELP1 protein expression. Mammary gland lysates from mice treated with or without doxycycline were subjected to immunoprecipitation with anti-T7 antibody followed by detection of PELP1 by Western blotting with the anti-T7 antibody. C, Spatial regulation of transgene expression was confirmed by using IHC for the T7 tag of PELP1 and the epithelial surface marker cytokeratin-8 (CK 8).