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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Invest Dermatol. 2014 Jul 9;135(1):299–301. doi: 10.1038/jid.2014.287

Figure 2. Effects of DLX3 deletion in the infundibulum/isthmus on hair differentiation.

Figure 2

(a) Time schedule of tamoxifen treatment and depilation applied on DLX3K14CreERT cKO mice. Gross appearance of hair pelage (right side: depilated) and hematoxylin/eosin staining of skin sections (depilated). Scale bar: 100 μm.

(b) Immunohistochemical analysis of DLX3 (red) in depilated skin samples. DAPI (blue) marks nuclei. Scale bar: 50 μm.

(c) Detection of type I hair keratins (AE13) and trichohyalin (AE15) in hair follicle bulbs from depilated skin at PD6 and PD14. K17 expression in medulla and outer root sheath from depilated skin at PD14. Scale bar: 50 μm.

(d) Scanning electron microscopy analysis of depilated skin from DLX3K14CreERT cKO and control mice at PD14 showing hair structure. Lower panels show high resolution images of the cuticle in control and DLX3K14CreERT cKO mice. Scale bars: upper panels, 100 μm; lower panels, 25 μm.

(e) Detection of lipid deposit in the WT and DLX3K14CreERT cKO skin sections at telogen (P20). Red indicates Oil Red O staining. Scale bar: 50 μm.