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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Surg Res. 2014 Aug 20;193(2):745–753. doi: 10.1016/j.jss.2014.08.022

Figure 5. ITGB4 expression in 2D vs CTCs from the 4D model (H1299) and the effect on cell adhesion.

Figure 5

In the H1299 cells, the CTCs had significantly lower mRNA expression (A) and protein expression (B) than the same cells in the 2D culture. H1299 cells grown in the 2D culture treated with ITGB4 siRNA had significantly less mRNA (C) and less protein (D) than the cells treated with SCR. H1299 cells grown in the 2D culture treated with ITGB4 siRNA had significantly less adhesion to the petri dish than the same cells treated with SCR (E). H1299 cells grown in the 2D culture transfected with ITGB4 had significantly more mRNA (F) and more protein (G) than the cells transfected with the GFP vector. H1299 cells grown on the2D culture transfected with ITGB4 had significantly more adhesion to the petri dish than the same cells transfected with the GFP vector (H). The error bar represents the standard error of mean.