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. Author manuscript; available in PMC: 2015 Jun 11.
Published in final edited form as: Nature. 2014 Sep 28;516(7530):242–245. doi: 10.1038/nature13760

Figure 2. SVA elements are repressed by primate-specific ZNF91.

Figure 2

a, Relative luciferase activity of a SVA_D-SV40-luciferase reporter after co-transfection of KZNFs in mESCs. b, KAP1 and H3K4me3 ChIP-seq coverage tracks for a selection of loci in hESCs and TC11-mESCs transfected with EV or ZNF91. Pie charts: percentages of H3K4me3-positive SVAs on human chromosome 11. c, Median fold expression change (ZNF91/EV), for genes with (blue circles) or without (gray crosses) an SVA within the indicated genomic distance among the 994 expressed human chromosome 11 genes. d, ZNF91 structural evolution. Green stripes: duplicated zinc fingers; Blue stripes: zinc fingers that changed contact residues in the lineage to humans (dark blue) or in other lineages (light blue). Green arrows: segmental duplications. *= reconstructed ancestral protein. e, Relative SVA_D-SV40-luciferase activity in the presence of various ZNF91 proteins. Error bars: SEM; ** p<0.01