Table.
Comparison of characteristics of Walsh lab and Dorn lab cardiac Mfn2 knockout mouse findings.
| Walsh lab Mfn2 null1 | Dorn lab Mfn2 null2,3,4,5 | ||
|---|---|---|---|
| Adult (10–14 wks) | Juvenile (6 wks)2 | Mature (16–44 wks) | |
| Cardiac Phenotype | |||
| Mfn2 protein level | ↓90% | ↓80% | ↓80%3 |
| Ht weight/body wt | ↑20% | Nl | ↑35%4 |
| LV %FS | Nl | Nl | ↓30–50%3 |
| +dP/dtmax resting | Nl | Nl | ↓50%3 |
| +dP/dtmax peak | ↓33% (isoproterenol) | Nl | ↓70% (dobutamine)3 |
| Mitochondrial Phenotype | |||
| Mitochondrial size | ↑2-fold (TEM) | ↑50% (TEM) | ↑30% (flow cyto)4 |
| Respiration | Nl | Nl | ↓25%4 |
| Δψm | ↓30% | Nl | ↓25%4 |
| ROS production | n.d. | Nl | ↑3-fold4 |
| Ca2++ induced swelling | ↓25% | Nl | Nl4 |
| SR-mito tethering | Nl (mito-SR distance) | ↓30% (mito-SR contact length) | n.d. |
| Gene expression | |||
| ANP | ↑3-fold | ↑3-fold5 | ↑4-fold5 |
| BNP | ↑20% | ↑2-fold5 | ↑4-fold5 |
| Tfam | ↓30% | Nl5 | ↑2-fold5 |
| PGC1α | ↓25% | Nl5 | Nl5 |
| cytb | ↓15% | Nl5 | Nl5 |
| ndf | ↓25% | Nl5 | Nl5 |
1
Papanicolaou KN et al. Mol Cell Biol 31:1309–28, 2011
2
Chen Y et al. Circ Res 111:863–75, 2012
3
Chen Y and Dorn GW II Science 340:471–475, 2013
4
Song M et al. Circ Res 115:348–353, 2014
5
Chen Y et al. Antioxid Redox Signal 2014, in press (online)