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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Am J Reprod Immunol. 2014 Oct 24;73(1):22–35. doi: 10.1111/aji.12336

Figure 3. Regulation of IL-6 and IL-8 secretion by HBCs following treatment with LPS, PG and Poly (I:C).

Figure 3

HBCs were maintained without (control, Ctrl) or with 1μg/ml of LPS, PG, or Poly (I:C) (PIC) for 24 h in medium with (Panels A and D), or without (Panels B and E) serum. Levels of IL-6 and IL-8 secreted into conditioned media from three independent experiments were determined by ELISA and normalized to total cell protein. Time course of LPS effects (C and F) and response of HBCs to LPS and LBP (G and H) in medium without serum are shown from a single experiment representing 3 identically conducted ones. Results which are not normally distributed are presented as medians and percentiles; the lines inside the box indicate the median, the ends of the box describe the lower and upper quartiles, and the whiskers define the smallest and largest values (A and B). Normally distributed results are presented as a mean + SE (D and E).

*P<0.05 vs Ctrl; **P<0.001 vs Ctrl