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. Author manuscript; available in PMC: 2015 Dec 20.
Published in final edited form as: FEBS Lett. 2014 Nov 11;588(24):4625–4630. doi: 10.1016/j.febslet.2014.11.001

Figure 3. GST-pull down assays confirm ASI interacts with M10 titin.

Figure 3

GST-M10titin fusion protein was immobilized on glutathione agarose followed by addition of protein extracts made from E. coli expressing either MBP-ASI, MBP-ASIa, MBP-ASIb, or MBPASIc. Protein complexes were eluted and separated by SDS-PAGE and visualized using anti-MBP antibody. Strong interaction was detected between the M10 region of titin and ASI. That interaction is further localized to the final 103 amino acids encoded by ASIc. Very weak interaction between the first two fragments of ASI and M10titin was detected.