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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Nov 18;1852(1):166–174. doi: 10.1016/j.bbadis.2014.11.013

Figure 2. RNA activation-mediated overexpression of MALAT1 promoted CRC cell proliferation and invasion.

Figure 2

(A) Schematic locations of small activation RNA (saRNA) target sites relative to the transcription starting site of MALAT1 gene including CpG island, Ctrl (scramble control), P1 (saRNA targeted in MALAT1 promoter region −592 bp), P2 (saRNA targeted in MALAT1 promoter region −348 bp), M1 (saRNA targeted in MALAT1 mRNA +258 bp), M2 (saRNA targeted in MALAT1 mRNA +380 bp), M3 (saRNA targeted in MALAT1 mRNA +1474 bp), M4 (saRNA targeted in MALAT1 mRNA +2584 bp). (B–C) saRNA-induced MALAT1 expression. SW480 cells were transfected with scramble (Ctrl) or different saRNAs (P1, P2, m1–m4). MALAT1 expression were assessed by semi-quantitative analysis (B) and real-time PCR (C). GAPDH was used as an internal control. (D, E) SW620 and SW480 cells expressed an elevated level of MALAT1 due to RNA activation (RNAa) as detected by semi-quantitative (D) and real-time PCR (E). (F) RNAa-activated MALAT1 promoted CRC cell proliferation as measured by CCK-8 assay. (G) RNAa-activated MALAT1 promoted CRC cell invasion as determined by matrigel invasion chamber analysis. *P < 0.05, **P <0.01 compared to corresponding control cells (Ctrl) (n=3).