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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Invest Dermatol. 2014 Jul 23;135(1):202–211. doi: 10.1038/jid.2014.315

Figure 2.

Figure 2

Galectin-3 influences Tyrp-1 expression but not that of tyrosinase. [A] Cultured melanocytes derived from light skins were transfected with control shRNA [Control shRNA] or galectin-3 shRNA [Gal3 shRNA] and co-labeled by immunofluorescence for galectin-3 and Tyrp-1 or tyrosinase. AmouhiRNA transfectants (a & c), was reduced in the Gal3 shRNA tranfectants (d & f). In contrast, tyrosinase expression was similar in both the shRNA transfectants (g & i) and the Gal3 shRNA transfectants (j & l). Bars = 40 microns.

[B] Cultured melanocytes transfected with control (Mock) or three galectin-3 (305,306, & 308) shRNAs demonstrating a reduction inexpression of galectin-3 (ranging between 20–30%) and Tyrp-1 (ranging between 20–42%) by silencing.

[C] Cell lines represented in [B] demonstrating a concurrent reduction in the catalytic activity of human Tyrp-1.