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. Author manuscript; available in PMC: 2015 Dec 20.
Published in final edited form as: FEBS Lett. 2014 Nov 21;588(24):4825–4830. doi: 10.1016/j.febslet.2014.11.017

Fig. 2. siRNA-mediated knockdown of HDAC3 expression increases PDGF-induced NOR1 mRNA expression.

Fig. 2

Fig. 2

(A–B) RASMC were transiently transfected with HDAC1, HDAC2, HDAC3 or scrambled (scr) siRNA (50 nM) for 6 h, and further cultured overnight. Transfected cells were starved in 0.01% FBS in DMEM for 48 hours and stimulated with PDGF (25 ng/ml) or vehicle (PBS) for 2 or 6 h as indicated for mRNA analyses. The expression of HDAC1, HDAC2, HDAC3 and NOR1 was normalized to RPL13A and expressed as mean ± SEM fold increase over scr-siRNA-transfected vehicle-treated cells (*P < 0.05 vs. vehicle, #P < 0.05 vs. scr-siRNA).