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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Biomaterials. 2014 Nov 26;40:61–71. doi: 10.1016/j.biomaterials.2014.11.011

Figure 4.

Figure 4

ADSCs anastomose with the host vasculature and express endothelial markers. Composition 1 fibrin gels (A–F) or Composition 2 fibrin gels (G-1) containing DiI-labeled ADSCs (red) were subcutaneously implanted in mice and left in place for 7 days. Prior to the sacrifice of the animal, the animal received FITC-dextrans (green) via a tail vein injection. The ADSCs contained within Composition 1 fibrin gels formed clear, multi-branching structures (A–F) that were ADSC derived (DiI+, A, D), and contained FITC-dextrans within the lumens of these structures (C, E). Additionally, these structures were positive for the endothelial cell marker, CD31 (magenta) (B). (G–I) In contrast, very few ADSCs remained in the Composition 2 fibrin gels that were implanted and there was no evidence of FITC-dextran perfused, DiI+ vessels. Scale bar = 50 μm for all images. Nuclei are counterstained with DAPI (blue) in (C,I)