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. Author manuscript; available in PMC: 2014 Dec 17.
Published in final edited form as: J Mol Cell Cardiol. 2013 Aug 9;63:146–154. doi: 10.1016/j.yjmcc.2013.07.019

Figure 5. Knock-down of FZD7 and FRS2 promotes CM commitment.

Figure 5

siRNAs targeting to FZD7 and FRS2, as well as a non-specific control siRNA (siCTL) were transfected into MCP formed re-aggregates (A) or monolayers (B). Cells were harvested on day 12 and day 20 of differentiation, with CM ratio analyzed by FACS. (C) A representative image showing the dissecting of primitive heart (red dots included area) from mouse E8.5 embryo. (D) Transfection of pre-miR-1 mimic into primitive mouse heart cells and quantification of CM differentiation 10 days later. * p<0.05.