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. Author manuscript; available in PMC: 2015 Jun 11.
Published in final edited form as: Nature. 2014 Nov 26;516(7530):259–262. doi: 10.1038/nature13966

Extended Data Figure 2. Prediction of MapZ topology.

Extended Data Figure 2

a. MapZ topology (i.e. specification of the membrane spanning segments and their IN/OUT orientation relative to the membrane) was predicted by five different topology algorithms (SCAMPI-seq, SACAMPI-msa, PRODIV-TMHMM, PRO-TMHMM and OCTOPUS) using TOPCONS (http://topcons.net). ZPRED (green line) predicts the distance to the membrane centre of each amino acid and ΔG-scale (light blue) shows the predicted free energy of membrane insertion for a window of 21 amino acids centred around each position in the sequence. The trans-membrane span is indicated in grey. Prediction of cytoplasmic and extracellular localizations are shown in red and dark blue, respectively. b. Wide-field microscopy images of cells producing the C-terminal fusion of MapZ with GFP. GFP fluorescence (right panel) and Phase contrast images (left panel). Images are representative of experiments made in triplicate.