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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Med. 2014 Jul 27;20(8):919–926. doi: 10.1038/nm.3599

Figure 4.

Figure 4

BMAL1 regulates CXCL5 expression in CCSP positive cells. (a) Secretion of CXCL1, CXCL5 and TNF-α by cultured mouse club cells (representative of 3 independent trials). (b) Expression of Cxcl1, Cxcl2 and Cxcl5 by primary human normal bronchial epithelial cells in response to IL-1β stimulation (values normalized to expression levels in unstimulated epithelial cells, n = 3). (c) Localization of Cxcl5 expression in the lungs of CCSP-Bmal−/− and wild-type littermates at CT0 (n = 4) and CT12 (n = 3); two-way ANOVA and post hoc Bonferroni; arrowheads mark bronchioles where expression is low; scale bar, 5 mm). (d) Expression of Cxcl5 mRNA over circadian time in lung from CCSP-Bmal−/− mice (n = 4) and wild-type littermates (n = 4), normalized to wild-type littermate value at CT0. (e) Circulating levels of CXCL5 in plasma determined at two opposing time points by ELISA in CCSP-Bmal−/− (CT0: n = 3 and CT12: n = 4) and wild-type littermates (CT0: n = 4 and CT12: n = 5). (f,g) Neutrophil counts and chemokine levels in BAL from Cxcl5−/− mice exposed to LPS at two opposing time points, n = 3, one-way ANOVA and post hoc Bonferroni. Data are expressed as mean ± s.e.m and *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005.