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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Med. 2014 Jul 27;20(8):919–926. doi: 10.1038/nm.3599

Figure 5.

Figure 5

Endogenous glucocorticoid rhythms regulate rhythmic repression of Cxcl5. (a) The effects of LPS, glucocorticoids and GR over-expression on activity of the Cxcl5-luciferase promoter in RAT-1 cells, as assessed by luciferase activity (representative of 4 independent trials). (b) Concentration of circulating glucocorticoids (Cort) in intact (n = 5/time point) and adrenalectomized (ADX, n = 8/time point) C57Bl/6 mice across the circadian day; dotted line represents lower limit of detection. (c) LPS-induced neutrophilia in ADX mice (CT0: n = 11; CT12: n = 13) and intact counterparts (n = 12) (scale bar, 100 μm Student’s t-test with Bonferroni correction for multiple comparisons) (d) LPS induced CXCL5 production in ADX mice (CT0: n = 6; CT12: n = 7) and intact counterparts (CT0: n = 6; CT12: n = 5). (e,f) Chromatin immunoprecipitation (ChIP) analysis in CCSP-Bmal1−/− and littermate controls (WT) of GR binding and H3/K27Ac at CT0 and CT12 to a hypersensitive region on the Cxcl5 promoter (e) and the Glul promoter (f) (n = 3; two-way ANOVA and post hoc Bonferroni). Data are expressed as mean ± s.e.m and *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005.