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. 1972 Aug;69(8):2006–2010. doi: 10.1073/pnas.69.8.2006

Induction of a Reductive Pathway for Deoxyribonucleotide Synthesis during Early Embryogenesis of the Sea Urchin

John M Noronha 1,*, Gerald H Sheys 1, John M Buchanan 1
PMCID: PMC426856  PMID: 4626400

Abstract

Cell-free extracts of Arbacia eggs (Arbacia punctulata) apparently do not contain an enzymatic system for the reduction of ribonucleotides to deoxyribonucleotides. However, during an interval of 5 hr after fertilization at 23°, an enzymatic system is produced that is capable of catalyzing the reduction of CDP to dCDP in the presence of Mg2+, ethylenediaminetetraacetate, ATP, and a reducing agent, dithiothreitol. The activity is first seen about 1 hr after fertilization, and reaches a peak at about 5 hr. The appearance of the ribonucleotide reductase is prevented by the addition of emetine or puromycin, inhibitors of protein synthesis, to the cells before fertilization. Inclusion of actinomycin D in the cell suspension at a concentration sufficient to inhibit synthesis of messenger RNA does not appreciably affect the production of the enzyme activity. Preexisting, maternal RNA is thus used for synthesis of reductase. Ribonucleotide reductase may, therefore, represent the first example of an enzyme system absent in unfertilized eggs that is produced in response to fertilization.

Keywords: ribonucleotide reductase, embryogenesis, Arbacia punctulata, masked messenger RNA

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2006

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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