FIG. 1.

Surface electroporation facilitates the direct transfection of dendritic cells in dermal tissue. Shown is a high-powered (×63) confocal micrograph of a dendritic cell transfected with plasmid expressing GFP (green). The image is taken from a sectioned biopsy of guinea pig skin that had been treated with reporter plasmid, electroporated with the SEP device, and removed 6 hr posttreatment. The skin section was processed with Hoechst stain (blue) to identify nuclei. The dendritic cell was captured in the dermal layer. IMARIS software was used postcapture to produce a three-dimensional image of the transfected cell and the nuclei of surrounding cells.