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. 2014 Dec 17;4:7518. doi: 10.1038/srep07518

Table 2. Library design of affinity maturation library ZVEGFR2_2matlib.

  Positions
  9 10 11 13 14 17 18 24 25 27 28 32 35
Codons                          
AAA (Lys) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
AAC (Asn) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
ACT (Thr) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
ATC (Ile) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
ATG (Met) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
CAG (Gln) 1 70.3 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
CAT (His) 1 1.7 2.2 0.9 2.3 1 1.6 81.1 1.7 0.8 0.8 0.8 1.1
CCG (Pro) 0 0 0 0 0 0 0 0 0 0 0 0 0
CGT (Arg) 1 1.7 2.2 0.9 61.2 83.5 1.6 1.1 1.7 0.8 0.8 0.8 1.1
CTG (Leu) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
GAA (Glu) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
GAC (Asp) 1 1.7 2.2 84.2 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
GCT (Ala) 1 1.7 2.2 0.9 2.3 1 72.6 1.1 1.7 0.8 0.8 0.8 1.1
GGT (Gly) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 71.1 0.8 0.8 0.8 1.1
GTT (Val) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 86.9 1.1
TAC (Tyr) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 86.2 0.8 81.1
TCT (Ser) 1 1.7 61.8 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1
TGC (Cys) 0 0 0 0 0 0 0 0 0 0 0 0 0
TGG (Trp) 1 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 86.5 0.8 0.8 1.1
TTC (Phe) 82.4 1.7 2.2 0.9 2.3 1 1.6 1.1 1.7 0.8 0.8 0.8 1.1

The percentages of the codons used in each of the thirteen randomized library positions are indicated.