Table 2. Library design of affinity maturation library ZVEGFR2_2matlib.
Positions | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
9 | 10 | 11 | 13 | 14 | 17 | 18 | 24 | 25 | 27 | 28 | 32 | 35 | |
Codons | |||||||||||||
AAA (Lys) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
AAC (Asn) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
ACT (Thr) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
ATC (Ile) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
ATG (Met) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
CAG (Gln) | 1 | 70.3 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
CAT (His) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 81.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
CCG (Pro) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
CGT (Arg) | 1 | 1.7 | 2.2 | 0.9 | 61.2 | 83.5 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
CTG (Leu) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
GAA (Glu) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
GAC (Asp) | 1 | 1.7 | 2.2 | 84.2 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
GCT (Ala) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 72.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
GGT (Gly) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 71.1 | 0.8 | 0.8 | 0.8 | 1.1 |
GTT (Val) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 86.9 | 1.1 |
TAC (Tyr) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 86.2 | 0.8 | 81.1 |
TCT (Ser) | 1 | 1.7 | 61.8 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
TGC (Cys) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
TGG (Trp) | 1 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 86.5 | 0.8 | 0.8 | 1.1 |
TTC (Phe) | 82.4 | 1.7 | 2.2 | 0.9 | 2.3 | 1 | 1.6 | 1.1 | 1.7 | 0.8 | 0.8 | 0.8 | 1.1 |
The percentages of the codons used in each of the thirteen randomized library positions are indicated.