Figure 1. Generation of EpiSCs from E6.5 epiblast.

(A) E6.5 embryos were recovered from GOF18 transgenic mice. GFP-positive epiblasts were used for generating EpiSCs. (B) GFP-positive outgrowths spreading out from the epiblasts. (C) Until the third passage, only a few cells and colonies in the culture were GFP-positive. (D) Pure populations of GFP-positive cells were established after three rounds of FACS. Scale bars are 100 μm.