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. 2014 Dec 17;4:7477. doi: 10.1038/srep07477

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(A) The GFP-positive F4-EpiSCs were stained positive for Oct4, but were weakly stained by anti-Nanog antibody. (B) F4-EpiSCs showed a very weak alkaline phosphatase activity, thus demonstrating their primed pluripotent state. (C) Representative real-time RT-PCR analysis data of F4-EpiSCs (from 3 lines of single-cell clones) and control ESCs using mouse ESC-specific and EpiSC markers. (D) DNA methylation patterns of the promoter regions of Stella and Dppa5. (E) Analysis of the Oct4 enhancer activity in ESCs and F4-EpiSCs. Relative luciferase activity was normalized to the activity of the empty vector. Scale bars are 100 μm.