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. 2014 Dec 17;4:7035. doi: 10.1038/srep07035

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(A) Upper bands, representative data of RT-PCR for SPARC mRNA in 10 gastric cancer cell lines and a control gastric epithelial cell line GES-1. GAPDH was used as the internal reference for RNA integrity and reverse transcription. Lower bands, protein expression of SPARC in 10 gastric cancer cell lines and the GES-1 cell line. (B) Representative data of MSP assay for methylation in SPARC gene in cell lines. PCR products using methylated primers and unmethylated primers were separated and visualized in 2% agarose gels. M = methylated band; U = unmethylated band. (C) Upper bands, representative data of RT-PCR for SPARC mRNA in gastric cancer cell lines before (−) and after (+) 5-Aza-Cdr treatment. Lower bands, protein expression of SPARC in gastric cancer cell lines and GES-1 cell line after 5-Aza-Cdr treatment.