Figure 9. Analysis of neuron-specific markers in mice with inducible oligodendrocyte ablation (oDTR) as compared with control mice.

(a–c) Immunohistochemistry with an antibody-recognizing FOX-3, a neuronal marker, indicates lack of change in neuronal density in DTX-treated oDTR mice as compared with corresponding control mice. spinal dorsal horn and surrounding white matter are shown in a and images in b represent high-magnification images from the grey matter region. The corresponding quantitative analysis of FOX-3-positive cells over an area of 22,500 μm² is shown in c. (d,e) DTX-treated oDTR mice show an early increase in APP expression, a marker of acute axonal injury via immunohistochemistry; higher-magnification view shown in lower panels. Corresponding quantification of APP immunoreactive axonal swellings over an area of 22,500 μm² is represented in e. Data were analysed via analysis of variance of random measures followed by post-hoc Fisher’s test; n=3 or 4 mice per group; *P<0.05. Scale bar, 300 μm in a, and d (upper row, low magnification), and 150 μm in b, and d (lower row, high magnification).