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. 2012 Oct 5;7(28):2165–2175. doi: 10.3969/j.issn.1673-5374.2012.028.001

Figure 2.

Figure 2

Examples of small (A, B, C) and large dorsal root ganglion (DRG) neurons (D, E, F) demonstrating the effects of incubation with enteric glia-conditioned medium.

(A) and (B) are differential interference contrast (DIC) images of single, dissociated small DRG neurons, and (D) and (E) are images of single dissociated large DRG neurons under a Nikon Eclipse 80i microscope.

(A) and (D) show typical neurons that had been cultured in neural basal medium. (B) and (E) show neurons that were cultured in enteric glia-conditioned medium. (C) and (F) show average neurite counts (± SEM) in DRG neurons (C, small; F, large) plotted against time.

Squares (+CDN) represent counts taken from DRG neurons incubated in enteric glia-conditioned medium, whereas diamonds (–CDN) represent counts taken from DRG neurons incubated in standard, unconditioned neurobasal medium. aP < 0.001, vs. –CDN. Neurite counts were analyzed using the Mann-Whitney U test with the Bonferroni correction applied. Scale bars: 30 μm for all of the images.