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. 2012 Oct 5;7(28):2165–2175. doi: 10.3969/j.issn.1673-5374.2012.028.001

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Enzyme-linked immunosorbent assay (ELISA) analysis of neurotrophic factor release of enteric glia (EG) cells.

EG cells were maintained in a standard culture medium containing 10% fetal bovine serum for 24 hours. Medium was changed to one with 2% fetal bovine serum, cells were cultured continually for another 48 hours, and then medium was removed and examined for neurotrophic factor concentration by ELISA.

EG cells released NGF, BDNF and GDNF under controlled conditions, with their respective concentrations reaching 250, 96.4 and 53.8 pg/mL respectively. All bars represent mean ± SEM of five independent experiments.

NGF: Nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell line-derived neurotrophic factor.