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. 2012 Oct 25;7(30):2333–2339. doi: 10.3969/j.issn.1673-5374.2012.30.002

Figure 4.

Figure 4

Effect of different doses of oxymatrine on lipopolysaccharide(LPS)-induced production of nuclear factor-κB (NF-κB) p65 in nucleus and phosphorylated IκBα (p-IκBα) in cytoplasm.

(A) NF-κB p65 protein (65-kDa) levels in the cerebral cortex of rat brains were detected by western blot assay. β-actin (43-kDa) was used as an internal control.

(B) p-IκBα protein (41-kDa) levels in the cerebral cortex of rat brains were detected by western blot analysis. β-actin (43-kDa) was used as an internal control. I–V: Sham, LPS, high, medium, low dose oxymatrine groups, respectively.

(C) Graph showing changes in the levels of NF-κB p65 in the nucleus and p-IκBα in the cytoplasm in the different groups. Data are expressed as mean ± SD (ratio of the absorbance value of NF-κB p65/p-IκBα to that of β-actin); there are three rats in each group. aP < 0.05, bP < 0.01, vs. sham-operated (Sham) group; cP < 0.05, vs. LPS group, one-way analysis of variance followed by a Scheffé test.