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. 2014 Dec 10;14:344. doi: 10.1186/s12870-014-0344-x

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© Gondolf et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cell wall monosaccharide composition analysis of stems from plants co-overexpressing At UGE2 and GalS1. The monosaccharide composition of stems from plants in the T3 generation expressing either p35S:GalS1 or pIRX5:NST1-UGE2 or co-overexpressing either pIRX5:NST1-UGE2/p35S:GalS1 or p35S:UGE2/p35S:GalS1 was determined. Monosaccharide levels are shown as molar percentage ± SD (n = 5). Significantly increased levels of galactose (p ≤ 0.01, indicated with asterisks) were found with the two co-expressing constructs pIRX5:NST1-UGE2/p35S:GalS1 and p35S:UGE2/p35S:GalS1, while no other sugars were different from empty vector control plants (EVC) in any of the transgenic plants (p > 0.05). Analysis in the T2 generation of three independent lines for each construct showed the same results. The data were analyzed by pairwise t-test with Holm-Bonferroni correction for family-wise error rate. GalA, α-d-galacturonic acid; GlcA, α-d-glucuronic acid.