Figure 6.

Construction of retinal mosaic maps. A. Single slice of a confocal laser scanning stack (acquired with a 25× objective), showing about half of all ommatidia, in the centre of a male D. simulans ZOM4 retina. p + OC ommatidia are distinguished by staining with anti-Rh3 (red) from y ommatidia stained with anti-Rh4 (yellow). Actin (blue) staining visualizes the rhabdomeres. A'. The equator (white dashed line) in the boxed region in A at higher magnification (40× objective). B. SEM micrograph of a ZOM4 male head with the approximate location of equator (solid line) and dorsal-ventral midline (dashed line) indicated. Anterior is to the left. Scale bars A and C: 100 μm, A': 10 μm.