Table 1.
Farm | Sample size |
Prevalence (%)
Cryptosporidium / Giardia |
Cryptosporidium species (no.) | Giardia duodenalis assemblage (no.) |
---|---|---|---|---|
1 | 45 | 2.22%/2.22% | C. bovis (1)* | B2 (1)* |
2 | 32 | 9.38%/6.25% | C. parvum (1)**, C. bovis (2)** | B2 (2)* |
3 | 57 | 3.51%/3.51% | C. parvum (2)*** | E (1), B1 (1)*** |
4 | 46 | 4.35%/6.52% | C. parvum (2) | E (3) |
5 | 63 | 3.17%/7.94% | C. parvum (2) | E (5) |
6 | 106 | 0.94%/1.89% | C. parvum (1) | E (2) |
7 | 110 | 6.36%/3.64% | C. parvum (7) | E (4) |
8 | 166 | 0/1.20% | E (2) | |
9 | 94 | 4.26%/3.19% | C. andersoni (4) | E (3) |
10 | 32 | 0/6.25% | E (2) | |
11 | 141 | 0/1.42% | E (2) | |
12 | 76 | 0/1.32% | E (1) | |
13 | 69 | 1.45%/0 | C. bovis (1) | |
14-20 | 329 | 0/0 | ||
Total | 1366 | 1.68%/2.12% | C. parvum (15), C. bovis (4), C. andersoni (4) |
The distributions of Cryptosporidium spp. and G. duodenalis assemblages were determined with PCR–RFLP analysis of the SSU rRNA gene and sequence analysis of the tpi and gdh genes.
*Sample positive for a mixed infection of C. parvum and G. duodenalis subtype B2.
**One sample showed a mixed infection of C. parvum and G. duodenalis subtype B2, and another sample showed a mixed infection of C. bovis and G. duodenalis subtype B2.
***Sample positive for a mixed infection of C. parvum and G. duodenalis subtype B1.