Table 1.
Anaerobic growth of Magnetospirillum sp. strain pMbN1 with binary mixtures of aliphatic and aromatic acids
| Binary mixture of adaptation and co-substrate | ||||||
|---|---|---|---|---|---|---|
| Phase 1 | Phase 2 | Phase 3 | ||||
| Adaptation substrate | Co-substrate | OD max | μ max (h −1 ) | Duration of diauxic lag phase (h) | OD max | μ max (h −1 ) |
| Cells adapted to succinate | ||||||
| Succinate (5 mM) | Benzoate (1 mM) | 0.30 | 0.19 | 2.1 ± 0.1 | 0.42 | 0.28 |
| Succinate (5 mM) | 4-Methylbenzoate (1 mM) | 0.61 | 0.24 | None | None | None |
| Succinate (5 mM) | 4-Hydroxybenzoate (1 mM) | 0.43 | 0.27 | 3.3 ± 0.1 | 0.51 | 0.50 |
| Succinate (5 mM) | Phenylacetate (1 mM) | 0.66 | 0.25 | None | None | None |
| Succinate (5 mM) | Acetate (8 mM) | 0.53 | 0.23 | None | None | None |
| Cells adapted to 4-methylbenzoate | ||||||
| 4-Methylbenzoate (1.5 mM) | Benzoate (1.5 mM) | 0.27 | 0.29 | 4.0 ± 0.0 | 0.41 | 0.29 |
| 4-Methylbenzoate (1.5 mM) | Phenylacetate (1.5 mM) | 0.30 | 0.29 | 5.0 ± 0.0 | 0.45 | 0.28 |
| Cells adapted to other aliphatic acids | ||||||
| Acetate (8 mM) | Benzoate (1 mM) | 0.38 | 0.18 | None | None | None |
| Acetate (8 mM) | 4-Methylbenzoate (1 mM) | 0.25 | 0.16 | 3.5 ± 0.0 | 0.32 | 0.24 |
| Pyruvate (6 mM) | Benzoate (1 mM) | 0.30 | 0.17 | 1.3 ± 0.3 | 0.54 | 0.37 |
| Pyruvate (6 mM) | 4-Methylbenzoate (1 mM) | 0.54 | 0.22 | None | None | None |
| Fumarate (5 mM) | Benzoate (1 mM) | 0.30 | 0.17 | 2.3 ± 0.0 | 0.43 | 0.29 |
| L-Malate (5 mM) | Benzoate (1 mM) | 0.31 | 0.44 | 2.6 ± 0.1 | 0.49 | 0.28 |
| Oxaloacetate (6 mM) | Benzoate (1 mM) | 0.31 | 0.19 | 1.0 ± 0.0 | 0.42 | 0.34 |
| Cells adapted to other aromatic acids | ||||||
| 4-Hydroxybenzoate (1 mM) | Succinate (5 mM) | 0.43 | 0.32 | 4.3 ± 0.0 | 0.49 | 0.50 |
Preferentially utilized substrates are highlighted in boldface. Applied substrate concentrations are indicated in parentheses. Values for maximal optical density (ODmax) and maximum specific growth rates (μmax) are based on three replicate cultures with standard deviations of below 5%. μmax was calculated from the slope of the active growth phase (m) according to μmax = m × 1/ΔOD. In the case of diauxic growth, phase 1 corresponds to the first active growth phase. In the case of monophasic growth, phase 1 corresponds to the only observed active growth phase. If present, phase 3 corresponds to a second active growth phase, with phase 2 representing the diauxic lag phase between phases 1 and 3 (Figure 1). For further details see Additional file 1: Figures S1–S3.