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Nanog colocalizes with Gata4 and FoxA2 in definitive endoderm cells. Cells were differentiated according to protocol described in Section 2 and analysed by confocal immunofluorescence (a) for Nanog, Gata4, and FoxA2 at day 8 of differentiation. (b) Analysis showing the percentage of cells coexpressing Nanog-Gata4 and Nanog-FoxA2 in D3 differentiated cells. Data are mean ± ESM from 7–9 fields.
