Figure 9.

Validation of RNA-seq results by RT-qPCR. Transcript levels of 10 randomly selected genes in the four samples were detected by RT-qPCR. A heat map (center) was constructed using mean values of transcript levels detected in three biological replicates. Normalized RPKM values of RNA-seq results are shown (left). For each gene, maximum transcript level in a certain sample was set to 100, and transcript levels in other samples are shown relative to the maximum level. Relative transcript levels are shown by color scales (right). R, correlation coefficient value between RNA-seq data and RT-qPCR data.