Figure 2.

GAD67 expression in the mouse vertical crista. (A) Schematic representation showing a representative slice (gray) of the crista ampullaris. Dots represent hair bundles protruding from the crista surface. E.C.: eminentia cruciata; P.S.: planum semilunatum. (B,C,D) Photomicrographs of a superficial confocal section of the slice schematized in (A) showing the cell nuclei (blue; DAPI), the cellular expression of GAD67 (green; GFP), and the merged image, respectively. Light blue indicates co-localization of nuclei and GFP, which seems poor because GAD67 is expressed in the cytoplasm. Most cell bodies at this confocal level appear sectioned transversally. (E) Photomicrograph of a lower confocal section of the same specimen (merged image). Most cell bodies appear now sectioned longitudinally, as inferred from their elongated shape.