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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Lasers Surg Med. 2014 Mar 18;46(4):290–301. doi: 10.1002/lsm.22231

Figure 4.

Figure 4

Effect of inhibition of necroptosis mediator RIPK1 on N1S1 and AS30D HCC cell viability following heat stress. (A) N1S1 and (B) AS30D HCC cells pre-treated with the RIPK1 inhibitor necrostatin-1 (30μM) or vehicle control (0.1% DMSO) for one hour followed by heat stress at the indicated temperatures ranging from 37°C–50°C for 10 minutes were assessed with WST-1 viability assay at 24 hours post-heat stress. Data were normalized to 37°C control and analyzed using one-way analysis of variance (ANOVA) followed by unpaired t-test. Data are presented as mean±SEM (N=3).