. Author manuscript; available in PMC: 2014 Dec 17.

Published in final edited form as: Mol Pharm. 2008 Aug 19;5(5):808–817. doi: 10.1021/mp800027f

Table 5.

Effect of excipients on fluorescent properties and antigenicity after exposure to Moisture (37 °C and 80% RH for 30 days)

	Fluorescence properties
Excipients	Soluble protein (%)	λ_max	I₃₅₀/I₃₂₉	Antigenicity (%)
TT standard		329	0.75	100
Lyophilized TT standard		327	0.73	100
None	15 ± 1 (15 d)	^*	^*	^*
Histidine	101 ± 5	329	0.72	24
Lysine	103 ± 1	329	0.76	81 ± 12
Trehalose	92 ± 4	328	0.73	47
Sorbitol	87 ± 1	329	0.75	90 ± 8
Monomers (pH 2.9)	Aggregates formed before incubation	^*	^*	^*
MgCO₃/monomers pH 5.7	9 ± 1	^*	^*	^*
MgCO₃/monomers pH 8	4 ± 2	^*	^*	^*

Not determined